Home  Guidelines for Reviewers About the Journal News Editorial Board Aims & Scope Subscription Contact us Announcement Peer Review Policy Content
Early Edition  //  Current Issue  //  Archives  //  Most Read

General Chemistry ›› 2016, Vol. 2 ›› Issue (2): 84-88.DOI: 10.21127/yaoyigc20160001

• Full Papers • Previous Articles    

Preparation of Poly Lactic-co-Glycolic Acid/Pigment Epithelium-Derived Factor Emulsions and Their Inhibitation on Vascular Endothelial Growth Factor Gene Expression

Junying Zhaoa,a, Yanji Zhua, Fan Yib, Jun Zhub*(), Xi Shenb*()   

  1. a Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Rui Jin Er Road, Shanghai 200025, China
    b National Engineering Research Center for Nanotechnology, 28 East Jiang Chuan Road, Shanghai 200241, China
  • Received:2016-03-19 Accepted:2016-04-11 Online:2016-07-06 Published:2016-04-12
  • Contact: Jun Zhu,Xi Shen


Pigment epithelium-derived factor (PEDF) is encapsulated in poly lactic-co-glycolic acid (PLGA) polymer to prepare PLGA/PEDF emulsions for high efficient inhibitation on vascular endothelial growth factor (VEGF) gene expression. Analyses by Fourier transform infrared spectrum (FT-IR), transmission electron microscopy (TEM) and dynamic light scattering (DLS) proved the formation of the PLGA/PEDF emulsions. Furthermore, CCK-8 assay was used to detect the cytotoxicity of the PLGA/PEDF emulsions to human umbilical vein endothelial cells (HUVEC). Finally, the VEGF levels in the HUVEC cell are tested by enzyme-linked immunosorbent assay at different conditions, such as different concentrations of PLGA/PEDF emulsion, the culture time and the hypoxia treatment. The results showed that the obtained PLGA/PEDF emulsions were good agents for controlled release and inhibit effectively VEGF expression under hypoxia. Therefore, proliferation of HUVEC cells was inhibited by PLGA/PEDF emulsions, and the cell viability of HUVEC cells decreased gradually with increase of concentrations of PLGA/PEDF emulsions.


Key words: pigment epithelium-derived factor, vascular endothelial growth factor level, hypoxia, cytotoxicity

Copyright © General Chemistry, All Rights Reserved.
Address: 425 East 76th Street, Apt 9E, New York, NY, 10021, United States