Abstract:

Perfluorotripropylamine (FC-3283), a kind of perfluorocarbon as oxygen carrier, is encapsulated in a copolymer using polyethylene glycol (PEG) to modify poly lactic-co-glycolic acid (PLGA) to prepare a PLGA-PEG/FC-3283 emulsion for highly efficient reoxygenation to cell. ¹H nuclear magnetic resonance (¹H NMR) spectra, Fourier transform infrared spectrum (FT-IR), transmission electron microscopy (TEM), dynamic light scattering (DLS) and high performance ion chromatography proved the formation of the PLGA-PEG and PLGA-PEG/FC-3283 emulsion. High performance ion chromatography revealed that FC-3283 content in the emulsion is 9.4%. Furthermore, CCK-8 assay was used to detect the cytotoxicity of the PLGA-PEG/FC-3283 emulsion to HCT 116 cells. Finally, the ability of oxygen supply and release of PLGA-PEG/FC-3283 emulsion was evaluated by CCK-8 asssy and fluorophotometry based on establishing hypoxia/reoxygenation model of HCT 116 cells with liquid paraffin. By detecting the cells increment rate and the content changes of reactive oxygen species (ROS) before and after reoxygenation, we explored the oxygen carry and release ability of PLGA-PEG/FC-3283 emulsion. The results showed that the cell viability increased significantly through PLGA-PEG/FC-3283 emulsion administration after the cells were treated with hypoxia.

Key words: Keywords perfluorocarbon emulsion, hypoxia/reoxygenation, cytotoxicity, reactive oxygen species