Abstract:

Ultrasound-mediated gene delivery is a kind of new method of gene delivery because ultrasound given a certain dose of irradiation can reversibly change the morphology of the cell membrane. Thus, a gene carrier material is obtained through a positive liposome filled with CO₂ gas and absorbed by DNA molecule, in which ultrasound-mediated gene delivery is established. Furthermore, the kind of new method of gene delivery is helpful for the dissociation of DNA molecules from liposome (LIP)/DNA complex and the cellular membrane permeability, which can increase SPCA-1 cell viability. Furthermore, the transfection efficiencies of LIP/DNA complex to cells are performed and the effect of ultrasonic time and intensity is further estimated.

Key words: positive liposome, ultrasound, gene delivery, transfection efficiency